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ITCH
(Plasmid #36197)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36197 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28-MHL (Addgene plasmid 26096)
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7325
  • Total vector size (bp) 8465
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ITCH
  • Alt name
    PDB: 3TUG
  • Alt name
    itchy E3 ubiquitin protein ligase homolog, HECT domain
  • Alt name
    AIF4, AIP4, dJ468O1.1, NAPP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1140
  • Mutation
    contains HECT domain
  • GenBank ID
    BC011571
  • Entrez Gene
    ITCH (a.k.a. ADMFD, AIF4, AIP4, NAPP1)
  • Tag / Fusion Protein
    • His tag with TEV cleavage (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ITCH was a gift from Cheryl Arrowsmith (Addgene plasmid # 36197 ; http://n2t.net/addgene:36197 ; RRID:Addgene_36197)