Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36264||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
Gene/Insert nameDynamin 2
SpeciesM. musculus (mouse)
Entrez GeneDnm2 (a.k.a. Dyn, Dyn2, Udnm, b2b2159C, b2b2159Clo)
- Promoter CMV
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
HA-tagged mouse dynamin 2 flanked by KpnI and XbaI sites. BamHI between N-terminal HA tag and beginning of dynamin coding seq.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HA-mDyn2 pcDNA3 was a gift from Pietro De Camilli (Addgene plasmid # 36264 ; http://n2t.net/addgene:36264 ; RRID:Addgene_36264)
For your References section:A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Ferguson SM, Brasnjo G, Hayashi M, Wolfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, Flavell R, Cremona O, Miesenbock G, Ryan TA, De Camilli P. Science. 2007 Apr 27;316(5824):570-4. 10.1126/science.1140621 PubMed 17463283