Addgene - pDDRFPA1-CaM Plasmid Data

Plasmid 36292: pDDRFPA1-CaM

Gene/insert name:
ddRFPA1-CaM
Vector backbone:
pcDNA3.1+
(Search Vector Database)
Backbone manufacturer:
Life Technologies
Vector type:
Mammalian Expression
Promoter:
CMV
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
TAATACGACTCACTATAGGG List of Sequencing Primers
3' sequencing primer:
TAGAAGGCACAGTCGAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Robert Campbell
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A fluorogenic red fluorescent protein heterodimer. Alford et al (Chem Biol. 2012 Mar 23;19(3):353-60. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36292" in your Materials and Methods section.