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pcDNA-ZapCY1
(Plasmid #36319)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    ZapCY1 high affinity Zn(II) sensor
  • Species
    Synthetic
  • Insert Size (bp)
    1626
  • GenBank ID
    HQ992986.1
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Zn(II) binding domain was obtained from a previously published sensor (PMID: 16720702).
  • Terms and Licenses

Depositor Comments

Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-ZapCY1 was a gift from Amy Palmer (Addgene plasmid # 36319)
  • For your References section:

    Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+ with genetically encoded sensors. Qin Y, Dittmer PJ, Park JG, Jansen KB, Palmer AE. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Epub 2011 Apr 18. 10.1073/pnas.1015686108 PubMed 21502528