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Plasmid 36319: pcDNA-ZapCY1
  • ZapCY1 high affinity Zn(II) sensor

  • 1626

  • Synthetic

  • HQ992986.1

  • pcDNA3.1(+)
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression

  • 5500

  • CMV

  • BamHI

  • No

  • EcoRI

  • No

  • T7 Forward List of Sequencing Primers

  • BGH Reverse

  • Ampicillin

  • DH10B

  • 37

  • High Copy

  • Neomycin

  • The Zn(II) binding domain was obtained from a previously published sensor (PMID: 16720702).

  • View sequences (2)
  • View map

  • Amy Palmer

  • UBMTA

Comments: 

Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+ with genetically encoded sensors. Qin et al (Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Epub 2011 Apr 18. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36319" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only