Addgene - pcDNA-ZapCY1 Plasmid Data

Plasmid 36319: pcDNA-ZapCY1

Gene/insert name:
ZapCY1 high affinity Zn(II) sensor
Insert size:
1626
Species:
Synthetic
GenBank ID:
HQ992986.1
Vector backbone:
pcDNA3.1(+)
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Promoter:
CMV
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 Forward List of Sequencing Primers
3' sequencing primer:
BGH Reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
The Zn(II) binding domain was obtained from a previously published sensor (PMID: 16720702).
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Amy Palmer
Terms and Licenses:
MTA

Comments:

Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+ with genetically encoded sensors. Qin et al (Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Epub 2011 Apr 18. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36319" in your Materials and Methods section.