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NHA-GW182
(Plasmid #36438)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 36438 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCl-neo_NHA
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 10624
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TNRC6A
  • Alt name
    GW182
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5124
  • Mutation
    aa 254-1962 only
  • Entrez Gene
    TNRC6A (a.k.a. CAGH26, GW1, GW182, TNRC6)
  • Tag / Fusion Protein
    • NHA (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

From depositor: "GW182 is a short isoform of TNRC6A. The NCBI only has TNRC6A long form sequence, which is overlapping with GW182 with additional 254 aa on N-terminal." See following PMID: 19056672

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NHA-GW182 was a gift from Edward Chan (Addgene plasmid # 36438)
  • For your References section:

    Divergent GW182 functional domains in the regulation of translational silencing. Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan EK. Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. 10.1093/nar/gkq1099 PubMed 21131274