Addgene - NHA-Delta 10 Plasmid Data

Plasmid 36925: NHA-Delta 10

Gene/insert name:
TNGW1
Alt name:
Delta 10
Insert size:
2067
Species:
H. sapiens (human)
GenBank ID:
KIAA1460
Fusion protein or tag:
NHA
Terminal:
N terminal on backbone
Mutation:
aa 566-1343 only
Vector backbone:
pCl-neo_NHA
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning method:
Gateway Cloning
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
EBV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Edward Chan
Terms and Licenses:
MTA

Comments:

There is a typo in the NAR paper that incorrectly lists the N-term truncation as AA655 (instead of the correct AA566).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Divergent GW182 functional domains in the regulation of translational silencing. Yao et al (Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36925" in your Materials and Methods section.