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NHA-Delta 10
(Plasmid #36925)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCl-neo_NHA
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    TNGW1
  • Alt name
    Delta 10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2067
  • Mutation
    aa 566-1343 only
  • GenBank ID
    KIAA1460
  • Tag / Fusion Protein
    • NHA (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

There is a typo in the NAR paper that incorrectly lists the N-term truncation as AA655 (instead of the correct AA566).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NHA-Delta 10 was a gift from Edward Chan (Addgene plasmid # 36925)
  • For your References section:

    Divergent GW182 functional domains in the regulation of translational silencing. Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan EK. Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. 10.1093/nar/gkq1099 PubMed 21131274