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pBT250_(pCA-G-intron(Neo)-tTA2)
(Plasmid #36876)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36876 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript SK(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 8144
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GFP
  • Alt name
    mut4EGFP
  • Mutation
    deleted nucleotides after nucleotide 274
  • Promoter CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    tTA2
  • Alt name
    tetracycline transactivator
  • Mutation
    removed start codon
  • Promoter CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    beta-globin intron
  • Mutation
    insertion of FRT, lox5171, lox2272, FRT, loxP-Neo-loxP
  • Promoter CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    Neo
  • Alt name
    neomycin phosphotransferase
  • Alt name
    neomycin resistance gene
  • Promoter SV40

Cloning Information for Gene/Insert 4

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT250_(pCA-G-intron(Neo)-tTA2) was a gift from Liqun Luo (Addgene plasmid # 36876 ; http://n2t.net/addgene:36876 ; RRID:Addgene_36876)
  • For your References section:

    Extensions of MADM (Mosaic Analysis with Double Markers) in Mice. Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H, Chen-Tsai Y, Luo L. PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27. 10.1371/journal.pone.0033332 PubMed 22479386