Purpose(Empty Backbone) This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20011||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- Flag (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII, XbaI, XhoI (not destroyed)
- 3′ cloning site KpnI, BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
This pcDNA3 plasmid contains a new poly-linker and single c-terminal flag-tag sequence for the expression of NF-kBs in mammalian cell lines. See map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:c-Flag pcDNA3 was a gift from Stephen Smale (Addgene plasmid # 20011 ; http://n2t.net/addgene:20011 ; RRID:Addgene_20011)
For your References section:A c-Rel subdomain responsible for enhanced DNA-binding affinity and selective gene activation. Sanjabi S, Williams KJ, Saccani S, Zhou L, Hoffmann A, Ghosh G, Gerondakis S, Natoli G, Smale ST. Genes Dev. 2005 Sep 15. 19(18):2138-51. 10.1101/gad.1329805 PubMed 16166378