Addgene - YWHAG Plasmid Data

Plasmid 36903: YWHAG

Gene/insert name:
YWHAG
Alt name:
PDB: 3UZD
Alt name:
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (14-3-3gamma)
Insert size:
786
Species:
H. sapiens (human)
Entrez Gene:
YWHAG (14-3-3GAMMA)
Fusion protein or tag:
6xHis with TEV Cleavage
Terminal:
C terminal on backbone
Vector backbone:
pTvHR21-SGC
(Search Vector Database)
Backbone manufacturer:
SGC Oxford
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
7387
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
unknown List of Sequencing Primers
3' sequencing primer:
unknown
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

PDB: 3UZD. http://www.thesgc.org/structures/structure_desc...
Codon optimized

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36903" in your Materials and Methods section.