Addgene - pCMMP-MCS-IRES-Puro Plasmid Data

Plasmid 36952: pCMMP-MCS-IRES-Puro

Gene/insert name:
None
Vector backbone:
pCMMP
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size (bp):
7037
Modifications to Backbone:
MCS (AgeI-HpaI-BstBI-NotI-XhoI-BamHI), IRES, Puro
Promoter:
CMV
5' sequencing primer:
pLXSN_5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (3)
Principal Investigator:
Bill Sugden
Terms and Licenses:
MTA

Comments:

Sugden lab plasmid p3048

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The LMP1 oncogene of EBV activates PERK and the unfolded protein response to drive its own synthesis. Lee et al (Blood. 2008 Feb 15;111(4):2280-9. Epub 2007 Nov 27. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36952" in your Materials and Methods section.