Addgene - pClneoFH human RASSF1A Plasmid Data

Plasmid 37016: pClneoFH human RASSF1A

Gene/insert name:
rassf1A
Species:
H. sapiens (human)
GenBank ID:
NP_009113
Entrez Gene:
RASSF1 (123F2, NORE2A, RASSF1A, RDA32, REH3P21)
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Vector backbone:
pCl-neo (modified)
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression
Modifications to Backbone:
pCI-neo vector was modified to include FLAG and His tags N-terminal to MCS. The resulting vector was named pCIneoFLAG-His6(FH).
Promoter:
CMV
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Yutaka Hata
Terms and Licenses:
MTA

Comments:

pCIneoFH vector has the sequence NheI-FLAG-His6-EcoRI-MluI-XbaI-SalI-SmaI-NotI.

A digest with NheI and SalI will excise the insert with tags.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Hippo pathway-dependent and -independent roles of RASSF6. Ikeda et al (Sci Signal. 2009 Sep 29;2(90):ra59. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37016" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

12239: pLVTH-sip53

11653: pLVUHshp53

12203: pJ3H-Mst1