pClneoFH human RASSF1A
(Plasmid #37016)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCl-neo (modified)
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    pCI-neo vector was modified to include FLAG and His tags N-terminal to MCS. The resulting vector was named pCIneoFLAG-His6(FH).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    rassf1A
  • Species
    H. sapiens (human)
  • GenBank ID
    NP_009113
  • Entrez Gene
    RASSF1 (a.k.a. 123F2, NORE2A, RASSF1A, RDA32, REH3P21)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

pCIneoFH vector has the sequence NheI-FLAG-His6-EcoRI-MluI-XbaI-SalI-SmaI-NotI.

A digest with NheI and SalI will excise the insert with tags.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClneoFH human RASSF1A was a gift from Yutaka Hata (Addgene plasmid # 37016)
  • For your References section:

    Hippo pathway-dependent and -independent roles of RASSF6. Ikeda M, Kawata A, Nishikawa M, Tateishi Y, Yamaguchi M, Nakagawa K, Hirabayashi S, Bao Y, Hidaka S, Hirata Y, Hata Y. Sci Signal. 2009 Sep 29;2(90):ra59. 10.1126/scisignal.2000300 PubMed 19797269