pClneoFH human RASSF1A
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37016||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepCl-neo (modified)
Modifications to backbonepCI-neo vector was modified to include FLAG and His tags N-terminal to MCS. The resulting vector was named pCIneoFLAG-His6(FH).
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneRASSF1 (a.k.a. 123F2, NORE2A, RASSF1A, RDA32, REH3P21)
- Promoter CMV
/ Fusion Proteins
- FLAG (N terminal on backbone)
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer T3 (Common Sequencing Primers)
pCIneoFH vector has the sequence NheI-FLAG-His6-EcoRI-MluI-XbaI-SalI-SmaI-NotI.
A digest with NheI and SalI will excise the insert with tags.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pClneoFH human RASSF1A was a gift from Yutaka Hata (Addgene plasmid # 37016)
For your References section:Hippo pathway-dependent and -independent roles of RASSF6. Ikeda M, Kawata A, Nishikawa M, Tateishi Y, Yamaguchi M, Nakagawa K, Hirabayashi S, Bao Y, Hidaka S, Hirata Y, Hata Y. Sci Signal. 2009 Sep 29;2(90):ra59. 10.1126/scisignal.2000300 PubMed 19797269