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Plasmid 37016: pClneoFH human RASSF1A
  • rassf1A

  • H. sapiens (human)

  • NP_009113

  • RASSF1 (123F2, NORE2A, RASSF1A, RDA32, REH3P21)

  • FLAG

  • N terminal on backbone

  • His

  • N terminal on backbone

  • pCl-neo (modified)
    (Search Vector Database)

  • Promega

  • Mammalian Expression

  • pCI-neo vector was modified to include FLAG and His tags N-terminal to MCS. The resulting vector was named pCIneoFLAG-His6(FH).

  • CMV

  • EcoRI

  • No

  • SalI

  • No

  • CMV-F List of Sequencing Primers

  • T3

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • Yutaka Hata

  • UBMTA

Comments: 

pCIneoFH vector has the sequence NheI-FLAG-His6-EcoRI-MluI-XbaI-SalI-SmaI-NotI.

A digest with NheI and SalI will excise the insert with tags.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Hippo pathway-dependent and -independent roles of RASSF6. Ikeda et al (Sci Signal. 2009 Sep 29;2(90):ra59. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37016" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only