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Lenti-Hb9-GFP
(Plasmid #37080)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37080 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    CSCspPW
  • Backbone manufacturer
    Inder Verma lab
  • Backbone size w/o insert (bp) 8466
  • Total vector size (bp) 12486
  • Modifications to backbone
    none
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    none
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hb9 GFP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4020
  • Promoter Hb9

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GCTAGCTCCGGAGAGCAAAAGCTG
  • 3′ sequencing primer TGTAATCCAGAGGTTGATTG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Expresses GFP under the control of the Hb9 promoter to visualize postmitotic human motor neurons

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-Hb9-GFP was a gift from Fred Gage (Addgene plasmid # 37080 ; http://n2t.net/addgene:37080 ; RRID:Addgene_37080)
  • For your References section:

    Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor neurons derived from human embryonic stem cells. Marchetto MC, Muotri AR, Mu Y, Smith AM, Cezar GG, Gage FH. Cell Stem Cell. 2008 Dec 4;3(6):649-57. 10.1016/j.stem.2008.10.001 PubMed 19041781