pHB9-EGFP (Hb9 promoter) [TJ#96]
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16275||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeMammalian Expression, Mouse Targeting
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHB9 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)9000
/ Fusion Protein
- EGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
Approximately 9 kb fragment comprising the 5' upstream region of the murine HB9 gene, followed by a 5' splice substrate, an eGFP gene, and a bovine growth hormone polyadenylation signal.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHB9-EGFP (Hb9 promoter) [TJ#96] was a gift from Thomas Jessell (Addgene plasmid # 16275 ; http://n2t.net/addgene:16275 ; RRID:Addgene_16275)
For your References section:Conditional rhythmicity of ventral spinal interneurons defined by expression of the Hb9 homeodomain protein. Wilson JM, Hartley R, Maxwell DJ, Todd AJ, Lieberam I, Kaltschmidt JA, Yoshida Y, Jessell TM, Brownstone RM. J Neurosci. 2005 Jun 15. 25(24):5710-9. 10.1523/JNEUROSCI.0274-05.2005 PubMed 15958737