|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37085||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerK. Deisseroth Lab
- Backbone size w/o insert (bp) 5613
- Total vector size (bp) 7260
Vector typeAAV ; r
Growth in Bacteria
Insert Size (bp)846
- Promoter EF1a
- Cloning method Restriction Enzyme
- 5′ cloning site Asc1 (not destroyed)
- 3′ cloning site Nhe1 (not destroyed)
- 5′ sequencing primer CTTCCATTTCAGGTGTCGTG
- 3′ sequencing primer GCAGCGTATCCACATAGCG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byKarl Deisseroth Lab, Stanford Univ.
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-Ef1a-DO-EGFP-WPRE-pA was a gift from Bernardo Sabatini (Addgene plasmid # 37085 ; http://n2t.net/addgene:37085 ; RRID:Addgene_37085)
For your References section:Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression in neurons. Saunders A, Johnson CA, Sabatini BL. Front Neural Circuits. 2012 Jul 27;6:47. doi: 10.3389/fncir.2012.00047. eCollection 2012. 10.3389/fncir.2012.00047 PubMed 22866029