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Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression in neurons.
Saunders A, Johnson CA, Sabatini BL
Front Neural Circuits. 2012 Jul 27;6:47. doi: 10.3389/fncir.2012.00047. eCollection 2012.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
37083pAAV-Ef1a-DIO-mCherry-WPRE-pACre-dependent expression of mCherry
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37084pAAV-Ef1a-DIO-EGFP-WPRE-pACre-dependent expression of EGFP
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37085pAAV-Ef1a-DO-EGFP-WPRE-pAExpresses EGFP in cells lacking Cre (Cre-Off)
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37086pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pAExpresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitation
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37087pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pAExpresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibition
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37088pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pAExpresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic inhibition
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37089pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pAExpresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation
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37090pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pAExpresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation
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37091pAAV-Ef1a-FAS-EGFP-WPRE-pAExpresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)
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37092pAAV-Ef1a-FAS-TdTomato-WPRE-pAExpresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)
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37119pAAV-Ef1a-DO-mCherry-WPRE-pAExpresses mCherry in cells lacking Cre (Cre-Off)
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37120pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pADO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells
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37755pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pACre-dependent expression of ChETA-tdTomato, for optogenetic excitation
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37756pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pAExpresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for optogenetic excitation
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Antibodies from Article