Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||37161||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)E. Coli VJS6737
Copy numberHigh Copy
Gene/Insert namercoM2 in pEXT20
Entrez GeneBxe_C0028 (a.k.a. Bxe_C0028)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pEXT20-F2 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This strain contains Roberts lab plasmid pUX2397 which contains an EcoRI-HindIII fragment bearing Burkholderia xenovorans LB400 rcoM2 cloned into pEXT20. Suitable for expression of Burkholderia xenovorans RcoM2 protein (untagged). Host strain is E. coli VJS6737.
For strain usage see attached pdf.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UQ4884 was a gift from Gary Roberts (Addgene plasmid # 37161)
For your References section:RcoM: a new single-component transcriptional regulator of CO metabolism in bacteria. Kerby RL, Youn H, Roberts GP. J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7. 10.1128/JB.00033-08 PubMed 18326575