Addgene - UQ4884 Plasmid Data

Bacterial strain 37161: UQ4884

Gene/insert name:
rcoM2 in pEXT20
Species:
Burkholderia xenovorans
Entrez Gene:
Bxe_C0028 (Bxe_C0028)
Vector backbone:
pEXT20
(Search Vector Database)
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
pEXT20-F2 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
E. Coli VJS6737
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Supplemental document:
Strains for production of RcoMBx-1 proteins and assay of in vivo activity (application/pdf)
Principal Investigator:
Gary Roberts
Terms and Licenses:
MTA

Comments:

This strain contains Roberts lab plasmid pUX2397 which contains an EcoRI-HindIII fragment bearing Burkholderia xenovorans LB400 rcoM2 cloned into pEXT20. Suitable for expression of Burkholderia xenovorans RcoM2 protein (untagged). Host strain is E. coli VJS6737.

For strain usage see attached pdf.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: RcoM: a new single-component transcriptional regulator of CO metabolism in bacteria. Kerby et al (J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37161" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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