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Plasmid 17727: pKM631
  • NusG

  • N-utilization substance G

  • 747

  • Aquifex aeolicus

  • NC_000918

  • nusG ()

  • MBP

  • N terminal on backbone

  • His6

  • C terminal on insert

  • pKM596
    (Search Vector Database)

  • Waugh lab

  • Bacterial Expression

  • 8264

  • n/a List of Sequencing Primers

  • n/a

  • Ampicillin

  • DH5alpha

  • 37

  • Low Copy

  • View sequences (2)
  • Notes from Addgene (1)

  • David Waugh

  • UBMTA

Comments: 

This vector produces an MBP-NusG-His6 fusion protein with a
recognition site for TEV protease (ENLYFQG) between MBP and NusG-His6.

See the Notes link from the Plasmid Links box for the amino acid sequence of the MBP-NusG-His fusion protein with TEV site.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: The P1' specificity of tobacco etch virus protease. Kapust et al (Biochem Biophys Res Commun. 2002 Jun 28. 294(5):949-55. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17727" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only