Addgene - pKM631 Plasmid Data

Plasmid 17727: pKM631

Gene/insert name:
NusG
Alt name:
N-utilization substance G
Insert size:
747
Species:
Aquifex aeolicus
GenBank ID:
NC_000918
Entrez Gene:
nusG ()
Fusion protein or tag:
MBP
Terminal:
N terminal on backbone
Fusion protein or tag:
His6
Terminal:
C terminal on insert
Vector backbone:
pKM596
(Search Vector Database)
Backbone manufacturer:
Waugh lab
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
8264
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
David Waugh
Terms and Licenses:
MTA

Comments:

This vector produces an MBP-NusG-His6 fusion protein with a
recognition site for TEV protease (ENLYFQG) between MBP and NusG-His6.

See the Notes link from the Plasmid Links box for the amino acid sequence of the MBP-NusG-His fusion protein with TEV site.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The P1' specificity of tobacco etch virus protease. Kapust et al (Biochem Biophys Res Commun. 2002 Jun 28. 294(5):949-55. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17727" in your Materials and Methods section.

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