|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17727||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerWaugh lab
- Backbone size w/o insert (bp) 8264
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Alt nameN-utilization substance G
Insert Size (bp)747
/ Fusion Proteins
- MBP (N terminal on backbone)
- His6 (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
This vector produces an MBP-NusG-His6 fusion protein with a
recognition site for TEV protease (ENLYFQG) between MBP and NusG-His6.
See the Notes link from the Plasmid Links box for the amino acid sequence of the MBP-NusG-His fusion protein with TEV site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKM631 was a gift from David Waugh (Addgene plasmid # 17727)
For your References section:The P1' specificity of tobacco etch virus protease. Kapust RB, Toezser J, Copeland TD, Waugh DS. Biochem Biophys Res Commun. 2002 Jun 28. 294(5):949-55. 10.1016/S0006-291X(02)00574-0 PubMed 12074568