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(Plasmid #17727)


Item Catalog # Description Quantity Price (USD)
Plasmid 17727 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Waugh lab
  • Backbone size w/o insert (bp) 8264
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
    N-utilization substance G
  • Species
    Aquifex aeolicus
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • His6 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector produces an MBP-NusG-His6 fusion protein with a
recognition site for TEV protease (ENLYFQG) between MBP and NusG-His6.

See the Notes link from the Plasmid Links box for the amino acid sequence of the MBP-NusG-His fusion protein with TEV site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM631 was a gift from David Waugh (Addgene plasmid # 17727 ; ; RRID:Addgene_17727)
  • For your References section:

    The P1' specificity of tobacco etch virus protease. Kapust RB, Toezser J, Copeland TD, Waugh DS. Biochem Biophys Res Commun. 2002 Jun 28. 294(5):949-55. 10.1016/S0006-291X(02)00574-0 PubMed 12074568