Addgene - pRK1037 Plasmid Data

Plasmid 8833: pRK1037

Gene/insert name:
TVMV protease, catalytic domain
Alt name:
tobacco vein mottling virus protease
Insert size:
700
Species:
tobacco vein mottling virus
Vector backbone:
pZA31
(Search Vector Database)
Backbone manufacturer:
Herman Bujard Lab
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
6700
Cloning site 5':
MfeI
Site destroyed during cloning:
Yes
Cloning site 3':
BglII
Site destroyed during cloning:
Yes
5' sequencing primer:
pLTet-F List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
BL21-Pro
Growth temperature (℃):
37
Growth instructions:
For best results, perform intracellular processing experiments at 30C.
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
David Waugh
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro. Nallamsetty et al (Protein Expr Purif. 2004 Nov . 38(1):108-15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8833" in your Materials and Methods section.

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