pRK1037
(Plasmid #8833)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pZA31
  • Backbone manufacturer
    Herman Bujard Lab
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21-Pro
  • Growth instructions
    For best results, perform intracellular processing experiments at 30C.
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    TVMV protease, catalytic domain
  • Alt name
    tobacco vein mottling virus protease
  • Species
    tobacco vein mottling virus
  • Insert Size (bp)
    700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MfeI (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer pLTet-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK1037 was a gift from David Waugh (Addgene plasmid # 8833)
  • For your References section:

    Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro. Nallamsetty S, Kapust RB, Tozser J, Cherry S, Tropea JE, Copeland TD, Waugh DS. Protein Expr Purif. 2004 Nov . 38(1):108-15. 10.1016/j.pep.2004.08.016 PubMed 15477088