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pEYFP-N1-A2BR
(Plasmid #37202)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37202 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEYFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5770
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A2BR
  • Alt name
    adenosine A2b receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1068
  • Entrez Gene
    ADORA2B (a.k.a. ADORA2)
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

A2B was PCR amplified as a 1068bp fragment incorporating a 5' EcoRI site. The stop codon was eliminated and a 3' BamHI site was added with the reverse PCR primer to allow direct cloning into pEYFP-N1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEYFP-N1-A2BR was a gift from Robert Tarran (Addgene plasmid # 37202 ; http://n2t.net/addgene:37202 ; RRID:Addgene_37202)
  • For your References section:

    Defective adenosine-stimulated cAMP production in cystic fibrosis airway epithelia: a novel role for CFTR in cell signaling. Watson MJ, Worthington EN, Clunes LA, Rasmussen JE, Jones L, Tarran R. FASEB J. 2011 Sep;25(9):2996-3003. Epub 2011 May 31. 10.1096/fj.11-186080 PubMed 21628448