pGEX-5X2-hDrp1(518-736)-Flag
(Plasmid #37244)

• Depositing Labs: Christopher Counter, David Kashatus
• Publication: Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15. doi: 10.1038/ncb2310.

Backbone

• Vector backbone: pGEX-5X2
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4973
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Drp1
• Alt name: DNM1L
• Species: H. sapiens (human)
• Mutation: contains AA 517-end
• GenBank ID: isoform 3 (NM_005690.3)
• Entrez Gene: DNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)
• Tags / Fusion Proteins:
  • FLAG (C terminal on insert)
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GST-F
• 3′ sequencing primer: pGEX_3_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results show a V638F mutation in pGEX-Drp1. Please note that this is a polymorphism that does not affect function.