Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed November 28th & 29th for the Thanksgiving holiday. Order processing and shipping may be delayed the week of November 25th - 29th. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pGEX-5X2-hDrp1(518-736)-Flag
(Plasmid #37244)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37244 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-5X2
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4973
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Drp1
  • Alt name
    DNM1L
  • Species
    H. sapiens (human)
  • Mutation
    contains AA 517-end
  • GenBank ID
    isoform 3 (NM_005690.3)
  • Entrez Gene
    DNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)
  • Tags / Fusion Proteins
    • FLAG (C terminal on insert)
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GST-F
  • 3′ sequencing primer pGEX_3_primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Addgene sequencing results show a V638F mutation in pGEX-Drp1. Please note that this is a polymorphism that does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-5X2-hDrp1(518-736)-Flag was a gift from Christopher Counter & David Kashatus (Addgene plasmid # 37244 ; http://n2t.net/addgene:37244 ; RRID:Addgene_37244)
  • For your References section:

    RALA and RALBP1 regulate mitochondrial fission at mitosis. Kashatus DF, Lim KH, Brady DC, Pershing NL, Cox AD, Counter CM. Nat Cell Biol. 2011 Aug 7;13(9):1108-15. doi: 10.1038/ncb2310. 10.1038/ncb2310 PubMed 21822277