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pYUB28b
(Plasmid #37277)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37277 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pYUB1049
  • Backbone manufacturer
    Ghader Bashiri
  • Backbone size (bp) 4921
  • Vector type
    Bacterial Expression ; mycobacteria shuttle vector
  • Promoter T7
  • Tags / Fusion Proteins
    • His-tag
    • His-tag

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYUB28b was a gift from Ted Baker (Addgene plasmid # 37277 ; http://n2t.net/addgene:37277 ; RRID:Addgene_37277)
  • For your References section:

    Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis. Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN. PLoS One. 2010 Dec 29;5(12):e15803. 10.1371/journal.pone.0015803 PubMed 21209917