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pDESTsmg
(Plasmid #18878)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18878 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pYUB1049
  • Backbone size (bp) 6573
  • Vector type
    Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    This is a Gateway destination vector, so it requires a ccdB survival strain of E. coli for propagation of plasmid.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was constructed by Rachael M. Goldstone from the pYUB1049 vector. pYUB1049 was donated by Mi-Sun Koo and William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA.
  • Terms and Licenses

Depositor Comments

This is a Gateway destination vector. It requires a ccdB survival strain of E. coli for propagation of the plasmid, but once a gene of interest has been cloned in (via the LR reaction) it will grow in standard E. coli strains (eg. DH5alpha, BL21).

This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning using E. coli.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDESTsmg was a gift from Ted Baker (Addgene plasmid # 18878 ; http://n2t.net/addgene:18878 ; RRID:Addgene_18878)
  • For your References section:

    A new Gateway vector and expression protocol for fast and efficient recombinant protein expression in Mycobacterium smegmatis. Goldstone RM, Moreland NJ, Bashiri G, Baker EN, Shaun Lott J. Protein Expr Purif. 2008 Jan . 57(1):81-7. 10.1016/j.pep.2007.08.015 PubMed 17949993