1
Plasmid 18878: pDESTsmg
  • None

  • His

  • N terminal on backbone

  • His

  • C terminal on backbone

  • pYUB1049
    (Search Vector Database)

  • Bacterial Expression

  • 6573

  • Gateway Cloning

  • T7 List of Sequencing Primers

  • Chloramphenicol

  • ccdB Survival

  • 37

  • This is a Gateway destination vector, so it requires a ccdB survival strain of E. coli for propagation of plasmid.

  • Unknown

  • Hygromycin

  • This plasmid was constructed by Rachael M. Goldstone from the pYUB1049 vector. pYUB1049 was donated by Mi-Sun Koo and William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA.

  • View sequences (2)
  • View map

  • Ted Baker

  • UBMTA

Comments: 

This is a Gateway destination vector. It requires a ccdB survival strain of E. coli for propagation of the plasmid, but once a gene of interest has been cloned in (via the LR reaction) it will grow in standard E. coli strains (eg. DH5alpha, BL21).

This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning using E. coli.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A new Gateway vector and expression protocol for fast and efficient recombinant protein expression in Mycobacterium smegmatis. Goldstone et al (Protein Expr Purif. 2008 Jan . 57(1):81-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18878" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only