Plasmid 18878: pDESTsmg

• Gene/insert name: None
• Fusion protein or tag: His
• Terminal: N terminal on backbone
• Fusion protein or tag: His
• Terminal: C terminal on backbone
• Vector backbone: pYUB1049
  (Search Vector Database)
• Vector type: Bacterial Expression
• Backbone size (bp): 6573
• Cloning method: Gateway Cloning
• 5' sequencing primer: T7
• Bacterial resistance(s) Chloramphenicol
• Growth strain(s) ccdB Survival
• Growth temperature (℃): 37
• Growth instructions: This is a Gateway destination vector, so it requires a ccdB survival strain of E. coli for propagation of plasmid.
• High or low copy: Unknown
• Selectable markers: Hygromycin
• Person or lab that originally
  cloned the gene/insert: This plasmid was constructed by Rachael M. Goldstone from the pYUB1049 vector. pYUB1049 was donated by Mi-Sun Koo and William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA.
• Sequence: View sequences (2)
• Map: View map
• Principal Investigator: Ted Baker
• Terms and Licenses: MTA

Comments: 

This is a Gateway destination vector. It requires a ccdB survival strain of E. coli for propagation of the plasmid, but once a gene of interest has been cloned in (via the LR reaction) it will grow in standard E. coli strains (eg. DH5alpha, BL21).

This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning using E. coli.

Article: A new Gateway vector and expression protocol for fast and efficient recombinant protein expression in Mycobacterium smegmatis. Goldstone et al (Protein Expr Purif. 2008 Jan . 57(1):81-7. PubMed)