Addgene - tandem PA-GFP-KanR Plasmid Data

Plasmid 37548: tandem PA-GFP-KanR

Gene/insert name:
tandem PA-GFP
Species:
S. cerevisiae (budding yeast)
Vector backbone:
pDH3
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4882
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
No
5' sequencing primer:
N/A List of Sequencing Primers
3' sequencing primer:
N/A
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers, other:
kanamycin
Sequence: View sequences (3)
Principal Investigator:
Wei-Lih Lee
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Photoactivatable GFP tagging cassettes for protein-tracking studies in the budding yeast Saccharomyces cerevisiae. Vorvis et al (Yeast. 2008 Sep;25(9):651-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37548" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

18820: pFA6a-PA-GFP-kanMX6

18822: pFA6a-PA-GFP-His3MX6

18821: pFA6a-PA-GFP-TRP1

18819: pDH3-PA-GFP