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GI-Gal4 DBD
(Plasmid #42500)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42500 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 9320
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GIGANTEA fused with Gal4 DBD
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    3981
  • Promoter CMV
  • Tag / Fusion Protein
    • Gal4 DNA binding domain (DBD) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GI-Gal4 DBD was a gift from Ricardo Dolmetsch (Addgene plasmid # 42500)
  • For your References section:

    Induction of protein-protein interactions in live cells using light. Yazawa M, Sadaghiani AM, Hsueh B, Dolmetsch RE. Nat Biotechnol. 2009 Oct;27(10):941-5. doi: 10.1038/nbt.1569. Epub 2009 Oct 4. 10.1038/nbt.1569 PubMed 19801976