Addgene - pCRY2PHR-mCherryN1 Plasmid Data

Plasmid 26866: pCRY2PHR-mCherryN1

Gene/insert name:
CRY2PHR-mCh
Alt name:
cryptochrome 2
Alt name:
mCherry
Insert size:
1507
Species:
A. thaliana (mustard weed)
Entrez Gene:
CRY2 (AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
Fusion protein or tag:
mCherry
Terminal:
C terminal on backbone
Vector backbone:
pmCherryN1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4722
Cloning site 5':
Xho I
Site destroyed during cloning:
No
Cloning site 3':
Xma I
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Chandra Tucker
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Rapid blue-light-mediated induction of protein interactions in living cells. Kennedy et al (Nat Methods. 2010 Oct 31. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26866" in your Materials and Methods section.