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pCIB1(deltaNLS)-pmGFP
(Plasmid #28240)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28240 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CIB1(deltaNLS)-pmGFP
  • Alt name
    CIB1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1003
  • Mutation
    NLS sequences mutated
  • Entrez Gene
    CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe I (not destroyed)
  • 3′ cloning site Age I (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIB1(deltaNLS)-pmGFP was a gift from Chandra Tucker (Addgene plasmid # 28240)
  • For your References section:

    Rapid blue-light-mediated induction of protein interactions in living cells. Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL. Nat Methods. 2010 Oct 31. ():. 10.1038/nmeth.1524 PubMed 21037589