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CRY2olig-mCherry
(Plasmid #60032)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60032 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherryN1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6187
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRY2PHR E490G
  • Alt name
    CRY2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1495
  • Mutation
    E490G
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRY2olig-mCherry was a gift from Chandra Tucker (Addgene plasmid # 60032)
  • For your References section:

    An optimized optogenetic clustering tool for probing protein interaction and function. Taslimi A, Vrana JD, Chen D, Borinskaya S, Mayer BJ, Kennedy MJ, Tucker CL. Nat Commun. 2014 Sep 18;5:4925. doi: 10.1038/ncomms5925. 10.1038/ncomms5925 PubMed 25233328