mCherry-CRY2clust
(Plasmid #105624)

• Purpose: Expresses improved CRY2PHR homo-oligomerization
• Depositing Lab: Won Do Heo
• Publication: Park et al Nat Commun. 2017 Jun 23;8(1):30. doi: 10.1038/s41467-017-00060-2.

Backbone

• Vector backbone: pmCherry-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4658
• Total vector size (bp): 6179
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry-CRY2PHR-9aa
• Alt name: mCherry-CRY2clust
• Alt name: 839529
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Insert Size (bp): 1521
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Promoter: CMV
• Tag / Fusion Protein:
  • 9 amino acids (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: MfeI (not destroyed)
• 5′ sequencing primer: ATGAAGATGGACAAAAAGACCATCGTCTGG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry