Addgene - MSCV-N E4orf4 Plasmid Data

Plasmid 38046: MSCV-N E4orf4

Gene/insert name:
E4 orf4
Alt name:
E4 orf 4
Alt name:
HAdV5 E4 orf4
Insert size:
342
Species:
Human adenovirus 5
GenBank ID:
AP_000229.1 AAW65532.1
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
MSCV-N FLAGHA
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
8162
Promoter:
PKG
Cloning method:
Gateway Cloning
5' sequencing primer:
LXSN 5' List of Sequencing Primers
3' sequencing primer:
MSCV-N seq. rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
M. Vidal lab
Sequence: View sequences (2)
Principal Investigator:
Karl Munger
Terms and Licenses:
MTA

Comments:

Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, adding several amino acid residues [STFLYKVVR] fused to the C-terminus of translated protein.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins. Rozenblatt-Rosen et al (Nature. 2012 Jul 26;487(7408):491-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 38046" in your Materials and Methods section.