YopH (residues 164-468)
PurposeThis plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79749||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepET co-transformation cloning vector (13S-A)
Backbone manufacturerScott Gradia (Addgene plasmid # 48323)
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameYopH (residues 164-468)
- Promoter T7
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YopH (residues 164-468) was a gift from John Chodera & Nicholas Levinson & Markus Seeliger (Addgene plasmid # 79749 ; http://n2t.net/addgene:79749 ; RRID:Addgene_79749)
For your References section:An open library of human kinase domain constructs for automated bacterial expression. Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD. Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081. 10.1021/acs.biochem.7b01081 PubMed 30004690