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pcDNA3 c-SRC (WT)
(Plasmid #42202)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42202 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 7000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-SRC
  • Alt name
    SRC
  • Alt name
    sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • Alt name
    p60-Src
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    L176F mutation compared to reference sequence NM_005417.3. See c-SRC supplementary sequence information file for more details
  • GenBank ID
    NM_005417.3 AAA60584.1
  • Entrez Gene
    SRC (a.k.a. ASV, SRC1, THC6, c-SRC, p60-Src)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

A 1.3kb HindIII-BamHI fragment and a 0.3kb BamHI-NotI fragment were subcloned from pBS/KS c-SRC into pcDNA3 via HindIII/NotI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 c-SRC (WT) was a gift from Robert Lefkowitz (Addgene plasmid # 42202 ; http://n2t.net/addgene:42202 ; RRID:Addgene_42202)
  • For your References section:

    Beta-arrestin-dependent formation of beta2 adrenergic receptor-Src protein kinase complexes. Luttrell LM, Ferguson SS, Daaka Y, Miller WE, Maudsley S, Della Rocca GJ, Lin F, Kawakatsu H, Owada K, Luttrell DK, Caron MG, Lefkowitz RJ. Science. 1999 Jan 29. 283(5402):655-61. 10.1126/science.283.5402.655 PubMed 9924018