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An open library of human kinase domain constructs for automated bacterial expression.
Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
79680MK14_HUMAN_D0This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79681VRK3_HUMAN_D0This plasmid encodes the kinase domain of VRK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79682GAK_HUMAN_D0This plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79683CSK_HUMAN_D0This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79684VRK1_HUMAN_D0This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79685KC1G3_HUMAN_D0This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79686FES_HUMAN_D0This plasmid encodes the kinase domain of FES. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79687PMYT1_HUMAN_D0This plasmid encodes the kinase domain of PMYT1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79688MK03_HUMAN_D0This plasmid encodes the kinase domain of MK03. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79689STK3_HUMAN_D0This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79690DYR1A_HUMAN_D0This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79691KC1G1_HUMAN_D0This plasmid encodes the kinase domain of KC1G1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79692MK11_HUMAN_D0This plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79693MK13_HUMAN_D0This plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79694EPHB1_HUMAN_D0This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79695MK08_HUMAN_D0This plasmid encodes the kinase domain of MK08. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79696CDK16_HUMAN_D0This plasmid encodes the kinase domain of CDK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79697EPHB2_HUMAN_D0This plasmid encodes the kinase domain of EPHB2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79698PAK4_HUMAN_D0This plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79699CDKL1_HUMAN_D0This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79700SRC_HUMAN_D0This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79701STK16_HUMAN_D0This plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79702MAPK3_HUMAN_D0This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79703PAK6_HUMAN_D0This plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79704CSK22_HUMAN_D0This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79705MERTK_HUMAN_D0This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79706PAK7_HUMAN_D0This plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79707CSK21_HUMAN_D0This plasmid encodes the kinase domain of CSK21. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79708EPHA3_HUMAN_D0This plasmid encodes the kinase domain of EPHA3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79709BMPR2_HUMAN_D0This plasmid encodes the kinase domain of BMPR2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79710M3K5_HUMAN_D0This plasmid encodes the kinase domain of M3K5. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79711KCC2G_HUMAN_D0This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79712E2AK2_HUMAN_D0This plasmid encodes the kinase domain of E2AK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79713MK01_HUMAN_D0This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79714CSKP_HUMAN_D0This plasmid encodes the kinase domain of CSKP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79715CHK2_HUMAN_D0This plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79716KC1G2_HUMAN_D0This plasmid encodes the kinase domain of KC1G2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79717DMPK_HUMAN_D0This plasmid encodes the kinase domain of DMPK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79718KCC2B_HUMAN_D0This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79719FGFR1_HUMAN_D0This plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79720KS6A1_HUMAN_D1This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79721DAPK3_HUMAN_D0This plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79722STK10_HUMAN_D0This plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79723KC1D_HUMAN_D0This plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79724KC1E_HUMAN_D0This plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79725NEK1_HUMAN_D0This plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79726CDK2_HUMAN_D0This plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79727ABL1_HUMAN_D0This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79728DAPK1_HUMAN_D0This plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79729DYRK2_HUMAN_D0This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79730HASP_HUMAN_D0This plasmid encodes the kinase domain of HASP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79731FGFR3_HUMAN_D0This plasmid encodes the kinase domain of FGFR3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79732EPHB3_HUMAN_D0This plasmid encodes the kinase domain of EPHB3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79733SLK_HUMAN_D0This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79734KCC2D_HUMAN_D0This plasmid encodes the kinase domain of KCC2D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79735NEK7_HUMAN_D0This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79736PHKG2_HUMAN_D0This plasmid encodes the kinase domain of PHKG2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79737VRK2_HUMAN_D0This plasmid encodes the kinase domain of VRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79738AAPK2_HUMAN_D0This plasmid encodes the kinase domain of AAPK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79739AURKA_HUMAN_D0This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79740MARK3_HUMAN_D0This plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79741KAPCA_HUMAN_D0This plasmid encodes the kinase domain of KAPCA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79742STK24_HUMAN_D0This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79743VGFR1_HUMAN_D0This plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79744KCC4_HUMAN_D0This plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79745KCC1G_HUMAN_D0This plasmid encodes the kinase domain of KCC1G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79746KCC2A_HUMAN_D0This plasmid encodes the kinase domain of KCC2A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79747FAK2_HUMAN_D0This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
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79748Lambda PhosphataseIntended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.
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79749YopH (residues 164-468)This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
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