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MK03_HUMAN_D0
(Plasmid #79688)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79688 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET His10 TEV LIC cloning vector (2B-T-10)
  • Backbone manufacturer
    Scott Gradia (Addgene plasmid # 78173)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MK03
  • Species
    H. sapiens (human)
  • Promoter T7
  • Tag / Fusion Protein
    • His10-TEV (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MK03_HUMAN_D0 was a gift from John Chodera & Nicholas Levinson & Markus Seeliger (Addgene plasmid # 79688 ; http://n2t.net/addgene:79688 ; RRID:Addgene_79688)
  • For your References section:

    An open library of human kinase domain constructs for automated bacterial expression. Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD. Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081. 10.1021/acs.biochem.7b01081 PubMed 30004690