pBA2257
(Plasmid
#38151)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 38151 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMal-C2
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Backbone manufacturerNew England Biolabls
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Modifications to backboneInserted Gateway Cloning Cassette in pMalC2 to create pKM596. Then inserted proMeCPA ORF by Gateway cloning.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsuse BL21(DE3) for expression
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMBP-proMeCPA fusion protein
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SpeciesM. anisopliae
- Promoter tac
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Tag
/ Fusion Protein
- maltose binding protein (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13_pUC_rev_primer
- 3′ sequencing primer M13_pUC_fwd_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBA2257 was a gift from David Waugh (Addgene plasmid # 38151 ; http://n2t.net/addgene:38151 ; RRID:Addgene_38151) -
For your References section:
Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3). Austin BP, Waugh DS. Protein Expr Purif. 2012 Mar;82(1):116-24. Epub 2011 Dec 14. 10.1016/j.pep.2011.11.015 PubMed 22197595
Map uploaded by the depositor.
