Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38236||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5000
Modifications to backboneVector promoter was removed and replaced with the genomic mouse Eef2 promoter.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
SpeciesM. musculus (mouse)
Insert Size (bp)2000
MutationThe first nucleotides of the 5' UTR were changed from CTCTTCC to CAGGAAG
Entrez GeneEef2 (a.k.a. Ef-2)
- Promoter Endogenous Eef2
- Cloning method Restriction Enzyme
- 5′ cloning site Nco1 (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer gggctcgctgggtcctaggct
- 3′ sequencing primer CATCCGTTTCCTTTGTTCTGG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIS1-Eef25UTR-TOPmut-renilla was a gift from David Sabatini (Addgene plasmid # 38236 ; http://n2t.net/addgene:38236 ; RRID:Addgene_38236)
For your References section:A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM. Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. 10.1038/nature11083 PubMed 22552098