Addgene - pIS1 Plasmid Data

Plasmid 12179: pIS1

Gene/insert name:
None
Vector backbone:
pIS1
(Search Vector Database)
Backbone manufacturer:
Bartel Lab
Vector type:
Mammalian Expression, Luciferase
Backbone size (bp):
4085
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
EBV rev primer (GTGGTTTGTCCAAACTCATC)
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

pIS1 was derived from pRL-TK by adding more cloning sites. The plasmid contains a renilla luciferase reporter driven by the HSV TK promoter. Search Addgene's vector database for more information on pRL-TK from Promega.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12179" in your Materials and Methods section.