Addgene - pIS0 Plasmid Data

Plasmid 12178: pIS0

Gene/insert name:
None
Vector backbone:
pIS0
(Search Vector Database)
Backbone manufacturer:
Bartel Lab
Vector type:
Mammalian Expression, Luciferase
Backbone size (bp):
5264
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
EBV rev primer (GTGGTTTGTCCAAACTCATC)
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
David Bartel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

The firefly luciferase vector was modified from pGL3 Control Vector (Promega), such that a short sequence containing multiple cloning sites (5'-AGCTCTATACGCGTCTCAAGCTTACTGCTAGC GT-3') was inserted into the XbaI site immediately downstream from the stop codon.
3'UTR segments of target genes can be inserted into this vector to test for their effects on mRNA stability.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: MicroRNA-directed cleavage of HOXB8 mRNA. Yekta et al (Science. 2004 Apr 23. 304(5670):594-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12178" in your Materials and Methods section.