Addgene - pKM263-EcPPXc Plasmid Data

Plasmid 38328: pKM263-EcPPXc

Gene/insert name:
E. coli polyphosphatase
Alt name:
PPX
Species:
Escherichia coli
Entrez Gene:
ppx (ECO111_3226)
Fusion protein or tag:
6xHIS
Terminal:
N terminal on backbone
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Fusion protein or tag:
TEV
Terminal:
N terminal on backbone
Mutation:
C-terminus of E. coli polyphosphatase (described as a polyphosphate binding domain) - amino acid 305 (nucleotide 913) until end.
Vector backbone:
pKM263
(Search Vector Database)
Backbone manufacturer:
K. Melcher
Vector type:
Bacterial Expression
Promoter:
T7
Cloning site 5':
NcoI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7 Terminator
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (3)
Principal Investigator:
Florian Freimoser
Terms and Licenses:
MTA

Comments:

See "Bolesch, D.G., Keasling, J.D., 2000. Polyphosphate binding and chain length recognition of Escherichia coli exopolyphosphatase. J. Biol. Chem. 275, 33814–33819" for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Specific localization of inorganic polyphosphate (poly P) in fungal cell walls by selective extraction and immunohistochemistry. Werner et al (Fungal Genet Biol. 2007 Sep;44(9):845-52. Epub 2007 Jan 20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 38328" in your Materials and Methods section.

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