PurposeIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38770||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5603
- Total vector size (bp) 6263
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nameModified DsRed-Express2
Insert Size (bp)675
- Promoter EF-1a
/ Fusion Proteins
- ER signal sequence (MGWSCIILFLVATATGAHS) (N terminal on backbone)
- myc (C terminal on backbone)
- ER retention signal (KDEL) (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer EF1a_fwd
- 3′ sequencing primer BGH_rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byCloned by Birka Hein in the lab of Stefan Hell.
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Vector for targeting E2-Crimson to the ER in mammalian cells.
Substitutions in E2-Crimson relative to DsRed-Express2 are: E32V, Q66F, V71A, V73I, K83L, L85Q, F118L, L150N, I161N, K163M, V175C, Y193H, S197Y
IMPORTANT NOTE: There is an 82-bp intron after the ER signal sequence. The signal sequence will be in frame with the final product.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEF.myc.ER-E2-Crimson was a gift from Benjamin Glick (Addgene plasmid # 38770 ; http://n2t.net/addgene:38770 ; RRID:Addgene_38770)
For your References section:A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling. Strack RL, Hein B, Bhattacharyya D, Hell SW, Keenan RJ, Glick BS. Biochemistry. 2009 Sep 8;48(35):8279-81. 10.1021/bi900870u PubMed 19658435