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(Plasmid #38770)


Item Catalog # Description Quantity Price (USD)
Plasmid 38770 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5603
  • Total vector size (bp) 6263
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Modified DsRed-Express2
  • Species
  • Insert Size (bp)
  • Promoter EF-1a
  • Tags / Fusion Proteins
    • ER signal sequence (MGWSCIILFLVATATGAHS) (N terminal on backbone)
    • myc (C terminal on backbone)
    • ER retention signal (KDEL) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer EF1a_fwd
  • 3′ sequencing primer BGH_rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Vector for targeting E2-Crimson to the ER in mammalian cells.

Substitutions in E2-Crimson relative to DsRed-Express2 are: E32V, Q66F, V71A, V73I, K83L, L85Q, F118L, L150N, I161N, K163M, V175C, Y193H, S197Y

IMPORTANT NOTE: There is an 82-bp intron after the ER signal sequence. The signal sequence will be in frame with the final product.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF.myc.ER-E2-Crimson was a gift from Benjamin Glick (Addgene plasmid # 38770 ; ; RRID:Addgene_38770)
  • For your References section:

    A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling. Strack RL, Hein B, Bhattacharyya D, Hell SW, Keenan RJ, Glick BS. Biochemistry. 2009 Sep 8;48(35):8279-81. 10.1021/bi900870u PubMed 19658435