Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39194||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Amp (50ug/mL)
Growth instructionsReducing the incubation temperature to 30°C may reduce toxicity to the bacteria and improve growth
Gene/Insert nameERK2-MEK1 Fusion
SpeciesH. sapiens (human), R. norvegicus (rat)
Insert Size (bp)2400
GenBank IDM64300 NM_002755
Entrez GeneMAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)
Entrez GeneMapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
- Promoter CMV
/ Fusion Protein
- Myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
rat ERK2 fused to human MEK1 with a linker in between
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-myc-ERK2-MEK1_fusion was a gift from Melanie Cobb (Addgene plasmid # 39194 ; http://n2t.net/addgene:39194 ; RRID:Addgene_39194)
For your References section:A constitutively active and nuclear form of the MAP kinase ERK2 is sufficient for neurite outgrowth and cell transformation. Robinson MJ, Stippec SA, Goldsmith E, White MA, Cobb MH. Curr Biol. 1998 Oct 22;8(21):1141-50. 10.1016/S0960-9822(07)00485-X PubMed 9799732