Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39222||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
SpeciesR. norvegicus (rat)
Insert Size (bp)1077
Entrez GeneMapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
- Promoter CMV
/ Fusion Protein
- Myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5-myc-Erk2 was a gift from Melanie Cobb (Addgene plasmid # 39222 ; http://n2t.net/addgene:39222 ; RRID:Addgene_39222)
For your References section:Hydrophobic as well as charged residues in both MEK1 and ERK2 are important for their proper docking. Xu Be, Stippec S, Robinson FL, Cobb MH. J Biol Chem. 2001 Jul 13;276(28):26509-15. Epub 2001 May 14. 10.1074/jbc.M102769200 PubMed 11352917