|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||114291||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert namerERK2 and hMEK1 proteins
SpeciesH. sapiens (human), R. norvegicus (rat)
Mutationrat ERK2 fused to human MEK1 with a linker in between. L4A mutations are in the human MEK1 NES
Entrez GeneMapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
- Cloning method Gateway Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
rat ERK2 fused to human MEK1 with a linker in between. L4A mutations are in the human MEK1 NES. Subcloned from Addgene plasmid #39197. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCWXPGR-pTF-ERK:MEK was a gift from Patrick Salmon (Addgene plasmid # 114291 ; http://n2t.net/addgene:114291 ; RRID:Addgene_114291)