Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39213||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameKudzu Isoprene Synthase
SpeciesSynthetic; Pueraria montana
Insert Size (bp)1719
MutationDeleted the predicted chloroplast transit peptide
- Promoter psbA2
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI, NcoI (not destroyed)
- 3′ cloning site XbaI, BamHI (not destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Bentley FK, Melis A (2012) Diffusion-based process for carbon dioxide uptake and isoprene emission in gaseous/aqueous two-phase photobioreactors by photosynthetic microorganisms. Biotech Bioeng 109:100-109
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBA2kIKmA2 was a gift from Anastasios Melis (Addgene plasmid # 39213 ; http://n2t.net/addgene:39213 ; RRID:Addgene_39213)
For your References section:Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Lindberg P, Park S, Melis A. Metab Eng. 2010 Jan;12(1):70-9. Epub 2009 Oct 13. 10.1016/j.ymben.2009.10.001 PubMed 19833224
Map uploaded by the depositor.