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pAAV-Ef1a-DIO-VMAT2-WPRE-pA
(Plasmid #39339)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39339 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA
  • Backbone manufacturer
    K. Deisseroth Lab
  • Backbone size w/o insert (bp) 5603
  • Total vector size (bp) 6314
  • Modifications to backbone
    None
  • Vector type
    AAV ; r

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Slc18a2
  • Alt name
    VMAT2 (vesicular monoamine transporter 2)
  • Alt name
    SVAT (synaptic vesicle amine transporter)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1584
  • Mutation
    Myc tag inserted in the first lumenal loop between Glycines 98 and 99
  • GenBank ID
    NM_172523.3
  • Entrez Gene
    Slc18a2 (a.k.a. 1110037L13Rik, 9330105E13, Vmat2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asc1 (not destroyed)
  • 3′ cloning site Nhe1 (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene was custom synthesized and cloned in the rAAV backbone as indicated
  • Terms and Licenses
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Ef1a-DIO-VMAT2-WPRE-pA was a gift from Bernardo Sabatini (Addgene plasmid # 39339 ; http://n2t.net/addgene:39339 ; RRID:Addgene_39339)
  • For your References section:

    Dopaminergic neurons inhibit striatal output through non-canonical release of GABA. Tritsch NX, Ding JB, Sabatini BL.. Nature. 2012 Oct 3. doi: 10.1038/nature11466. 10.1038/nature11466