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pcDNA6-ARID1A 1759-2285
(Plasmid #39476)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39476 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA6-V5/His.b
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5212
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ARID1A
  • Species
    H. sapiens (human)
  • Mutation
    expresses ARID1A aa1759-2285
  • GenBank ID
    NM_006015.4
  • Entrez Gene
    ARID1A (a.k.a. B120, BAF250, BAF250a, BM029, C1orf4, CSS2, ELD, MRD14, OSA1, P270, SMARCF1, hELD, hOSA1)
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-fwd, T7-promoter
  • 3′ sequencing primer BGH-rev, V5-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Encodes the C-terminus of ARID1A.

Note that although the nucleotide sequence cloned in this plasmid starts with aa 1746, the cloning strategy was designed so that aa 1746- 1758 will not be translated, as there is no ATG start site. The actual ATG start site is at aa 1759.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA6-ARID1A 1759-2285 was a gift from Ie-Ming Shih (Addgene plasmid # 39476 ; http://n2t.net/addgene:39476 ; RRID:Addgene_39476)
  • For your References section:

    ARID1A, a factor that promotes formation of SWI/SNF-mediated chromatin remodeling, is a tumor suppressor in gynecologic cancers. Guan B, Wang TL, Shih IeM. Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7. 10.1158/0008-5472.CAN-11-1562 PubMed 21900401