Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39476||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5212
Vector typeMammalian Expression
Growth in Bacteria
SpeciesH. sapiens (human)
Mutationexpresses ARID1A aa1759-2285
Entrez GeneARID1A (a.k.a. B120, BAF250, BAF250a, BM029, C1orf4, CSS2, ELD, MRD14, OSA1, P270, SMARCF1, hELD, hOSA1)
/ Fusion Proteins
- V5 (C terminal on backbone)
- His (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-fwd, T7-promoter
- 3′ sequencing primer BGH-rev, V5-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Encodes the C-terminus of ARID1A.
Note that although the nucleotide sequence cloned in this plasmid starts with aa 1746, the cloning strategy was designed so that aa 1746- 1758 will not be translated, as there is no ATG start site. The actual ATG start site is at aa 1759.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA6-ARID1A 1759-2285 was a gift from Ie-Ming Shih (Addgene plasmid # 39476 ; http://n2t.net/addgene:39476 ; RRID:Addgene_39476)
For your References section:ARID1A, a factor that promotes formation of SWI/SNF-mediated chromatin remodeling, is a tumor suppressor in gynecologic cancers. Guan B, Wang TL, Shih IeM. Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7. 10.1158/0008-5472.CAN-11-1562 PubMed 21900401