Addgene - pEGFP-gfap(Intron1/5'/Exon1-zebrafish) Plasmid Data

Plasmid 39761: pEGFP-gfap(Intron1/5'/Exon1-zebrafish)

Gene/insert name:
zebrafish gfap promoter- EGFP
Insert size:
7423
Species:
D. rerio (zebrafish)
GenBank ID:
NC_007114.5
Vector backbone:
pEGFP-1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
zebrafish expression
Backbone size w/o insert (bp):
4200
Promoter:
gfap
Cloning site 5':
Xho1
Site destroyed during cloning:
No
Cloning site 3':
Sal1
Site destroyed during cloning:
No
5' sequencing primer:
none List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Supplemental document:
zebrafish gfap:EGFP cloning details (application/vnd.openxmlformats-officedocument.wordprocessingml.d)
Principal Investigator:
Pamela Raymond
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: GFAP transgenic zebrafish. Bernardos et al (Gene Expr Patterns. 2006 Oct;6(8):1007-13. Epub 2006 Jun 9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 39761" in your Materials and Methods section.