pEGFP-gfap(Intron1/5'/Exon1-zebrafish)
(Plasmid #39761)

• Depositing Lab: Pamela Raymond
• Publication: Bernardos et al Gene Expr Patterns. 2006 Oct;6(8):1007-13. Epub 2006 Jun 9.

Backbone

• Vector backbone: pEGFP-1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4200
• Total vector size (bp): 11623
• Vector type: zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: zebrafish gfap promoter- EGFP
• Species: D. rerio (zebrafish)
• Insert Size (bp): 7423
• GenBank ID: NC_007114.5
• Entrez Gene: gfap (a.k.a. cb345, etID36982.3, gfapl, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485)
• Promoter: gfap

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: Sal1 (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • zebrafish gfap:EGFP cloning details
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry