Addgene - ChR2-2A-Halo Plasmid Data

Gene/insert name:
ChR2.GFP-2A-Halo.YFP
Alt name:
NpHR
Alt name:
channelrhodopsin
Alt name:
halorhodopsin
Insert size:
3356
Species:
C. reinhardtii, N. pharaonis
Fusion protein or tag:
GFP and YFP
Terminal:
C terminal on insert
Mutation:
Note that there is a S147R mut in YFP. This mutation is not known to affect fluorescence.
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
clontech
Vector type:
Mammalian Expression
Backbone size w/o insert:
3963
Cloning site 5':
Kpn1
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
AGCAGAGCTGGTTTAGTGAA List of Sequencing Primers
3' sequencing primer:
TAAAACCTCTACAAATGTGG
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Edward Boyden
Terms and Licenses:
MTA

Comments:

PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Informational lesions: optical perturbation of spike timing and neural synchrony via microbial opsin gene fusions. Han et al (Front Mol Neurosci. 2009 . 2():12. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22047" in your Materials and Methods section.