Gene/insert name: ChR2
Alt name: Channelrhodopsin-2
Insert size: 1000
Species: C. reinhardtii
Fusion protein or tag: GFP
Terminal: C terminal on insert
Vector backbone:
FCK(1.3)GW
(Search Vector Database )
Backbone manufacturer:
Pavel Osten
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9500
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
AgeI
Site destroyed during cloning:
No
5' sequencing primer:
na
List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
High or low copy:
Unknown
Sequence:
View sequences (3)
Map:
View map
Principal Investigator:
Edward Boyden
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
15814" in your Materials and Methods section.
PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS
REAGENT AND FURTHER REAGENTS IN THIS LINE.