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FCK-ChR2-GFP
(Plasmid #15814)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    FCK(1.3)GW
  • Backbone manufacturer
    Pavel Osten
  • Backbone size w/o insert (bp) 9500
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    ChR2
  • Alt name
    Channelrhodopsin-2
  • Species
    C. reinhardtii
  • Insert Size (bp)
    1000
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS
REAGENT AND FURTHER REAGENTS IN THIS LINE.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FCK-ChR2-GFP was a gift from Edward Boyden (Addgene plasmid # 15814)
  • For your References section:

    Millisecond-timescale, genetically targeted optical control of neural activity. Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. Nat Neurosci. 2005 Sep . 8(9):1263-8. 10.1038/nn1525 PubMed 16116447