|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15814||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerPavel Osten
- Backbone size w/o insert (bp) 9500
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsUse recombinase-free E. coli (e.g., Invitrogen's Stbl3)
Insert Size (bp)1000
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
PLEASE CONTACT ED BOYDEN (firstname.lastname@example.org) FOR DETAILS ON THIS
REAGENT AND FURTHER REAGENTS IN THIS LINE.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FCK-ChR2-GFP was a gift from Edward Boyden (Addgene plasmid # 15814)
For your References section:Millisecond-timescale, genetically targeted optical control of neural activity. Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. Nat Neurosci. 2005 Sep . 8(9):1263-8. 10.1038/nn1525 PubMed 16116447
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.