FCK-Halo-GFP
(Plasmid #14750)

• Purpose: Lentiviral expression of Halo-GFP for neuronal inhibition
• Depositing Lab: Edward Boyden
• Publication: Han et al PLoS One. 2007 Mar 21;2(3):e299.

Backbone

• Vector backbone: FCK(1.3)GW
• Backbone manufacturer: Pavel Osten
• Backbone size w/o insert (bp): 9500
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
• Alt name: Halo
• Alt name: Halo-GFP
• Alt name: NpHR
• Species: N. pharaonis
• Insert Size (bp): 1300
• Mutation: N. pharaonis halorhodopsin is mammalian codon-optimized
• Promoter: CaMKIIa
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: atgactgagaccctcccacccgtgactgaaagcgccgt

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We purchased the gene to be synthesized by Epoch Biolabs (Sugar Land, TX)
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE CONTACT ED BOYDEN () FOR DETAILS ON THIS
REAGENT AND FURTHER REAGENTS IN THIS LINE.