Addgene - FCK-Halo-GFP Plasmid Data

Plasmid 14750: FCK-Halo-GFP

Gene/insert name:
Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
Alt name:
Halo
Alt name:
Halo-GFP
Alt name:
NpHR
Insert size:
1300
Species:
N. pharaonis
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Mutation:
N. pharaonis halorhodopsin is mammalian codon-optimized
Vector backbone:
FCK(1.3)GW
(Search Vector Database)
Backbone manufacturer:
Pavel Osten
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9500
Cloning site 5':
AgeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
atgactgagaccctcccacccgtgactgaaagcgccgt List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
We purchased the gene to be synthesized by Epoch Biolabs (Sugar Land, TX)
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Edward Boyden
Terms and Licenses:
MTA

Comments:

PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS
REAGENT AND FURTHER REAGENTS IN THIS LINE.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. Han et al (PLoS ONE. 2007 . 2():e299. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14750" in your Materials and Methods section.