|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14750||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerPavel Osten
- Backbone size w/o insert (bp) 9500
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsUse recombinase-free E. coli (e.g., Invitrogen's Stbl3)
Gene/Insert nameMammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
Insert Size (bp)1300
MutationN. pharaonis halorhodopsin is mammalian codon-optimized
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer atgactgagaccctcccacccgtgactgaaagcgccgt (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS
REAGENT AND FURTHER REAGENTS IN THIS LINE.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FCK-Halo-GFP was a gift from Edward Boyden (Addgene plasmid # 14750 ; http://n2t.net/addgene:14750 ; RRID:Addgene_14750)
For your References section:Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. Han X, Boyden ES. PLoS ONE. 2007 . 2():e299. 10.1371/journal.pone.0000299 PubMed 17375185