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FCK-Halo-GFP
(Plasmid #14750)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    FCK(1.3)GW
  • Backbone manufacturer
    Pavel Osten
  • Backbone size w/o insert (bp) 9500
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
  • Alt name
    Halo
  • Alt name
    Halo-GFP
  • Alt name
    NpHR
  • Species
    N. pharaonis
  • Insert Size (bp)
    1300
  • Mutation
    N. pharaonis halorhodopsin is mammalian codon-optimized
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer atgactgagaccctcccacccgtgactgaaagcgccgt
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS
REAGENT AND FURTHER REAGENTS IN THIS LINE.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FCK-Halo-GFP was a gift from Edward Boyden (Addgene plasmid # 14750)
  • For your References section:

    Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. Han X, Boyden ES. PLoS ONE. 2007 . 2():e299. 10.1371/journal.pone.0000299 PubMed 17375185