Addgene - AAV-FLEX-Arch-GFP Plasmid Data

Gene/insert name:
Arch
Alt name:
Arch-GFP
Alt name:
archaerhodopsin-3
Alt name:
H. sodomense archaerhodopsin-3
Insert size:
1500
Species:
H. sodomense
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Mutation:
no
Vector backbone:
AAV-FLEX
(Search Vector Database)
Backbone manufacturer:
Scott Sternson
Vector type:
Mammalian Expression, Cre/Lox
Vector type:
adeno-associated virus
Backbone size w/o insert:
5000
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
BsrGI
Site destroyed during cloning:
No
5' sequencing primer:
CCCATAACTTCGTATAATGTATGC List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
Use recombinase-free E. coli (e.g., Invitrogen's Stbl3), grow at 30C
High or low copy:
Low Copy
Sequence: View sequences (3)
Map:
View map
Map:
View map
Principal Investigator:
Edward Boyden
Terms and Licenses:
MTA

Comments:

PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.

In the map below, the Selected Features list should be labeled as follows (instead of the 3 loxP sites listed from 3629-3692 and 5299-5403):

lox2272: 3629-3596 , loxp: 3725-3692

lox2272: 5266-5399 , loxp: 5370-5403

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: High-performance genetically targetable optical neural silencing by light-driven proton pumps. Chow et al (Nature. 2010 Jan 7. 463(7277):98-102. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22222" in your Materials and Methods section.